Anti-GLO1 antibodies are used in the immunodetection of the protein glyoxalase I. In humans, the canonical protein has a reported length of 184 amino acid residues and a mass of 20.8 kDa. Up to 2 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. A member of the Glyoxalase I protein family, GLO1 is known to catalyze the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Post-translational modifications have been described, including phosphorylation. Synonyms for this target antigen include GLYI, HEL-S-74, lactoylglutathione lyase, S-D-lactoylglutathione methylglyoxal lyase, aldoketomutase, and GLOD1. GLO1 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 60 citations in the literature describe the use of GLO1 antibodies in research. Western Blot is a widely used application for these antibodies. ELISA, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry are also common applications.