Anti-Carboxypeptidase A6 antibodies are used for the immunodetection of the protein encoded by the CPA6 gene. In humans, the canonical protein has a reported length of 437 amino acid residues and a mass of 51 kDa. Its subcellular localization is in the extracellular matrix and is secreted. Alternative splicing is reported to yield 3 different isoforms for this protein. It is reported to be expressed in the hippocampus, nucleus raphe, and cortex. A member of the Peptidase M14 protein family, it is suspected to be involved in the proteolytic inactivation of enkephalins and neurotensin in some brain areas. Post-translational modifications have been described, including glycosylation.
The carboxypeptidase A6 marker can be used to characterize Midbrain Splatter Neurons.* Other names for this target antigen include carboxypeptidase B. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Western Blot is the most common application for the Carboxypeptidase A6 antibodies listed below. ELISA and Immunohistochemistry are also common applications.
*HuBMAP Human Reference Atlas v1.4