Anti-WEE1 antibodies are used in the immunodetection of the protein WEE1 G2 checkpoint kinase. In humans, the canonical protein has a reported length of 646 amino acid residues and a mass of 71.6 kDa. Its subcellular localization is in the nucleus. Up to 2 different isoforms have been reported for this protein. It is notably expressed in many tissues, such as the gallbladder and nasopharynx. A member of the Ser/Thr protein kinase protein family, Wee1 is known to act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Post-translational modifications have been described, including ubiquitination and phosphorylation. Synonyms for this target antigen include WEE1hu, wee1-like protein kinase, WEE1 homolog, WEE1+ homolog, protein kinase, wee1A kinase, and WEE1A. WEE1 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.