Anti-CPA6 antibodies are used in the immunodetection of the protein carboxypeptidase A6. In humans, the canonical protein has a reported length of 437 amino acid residues and a mass of 51 kDa. Its subcellular localization is in the extracellular matrix and is secreted. Up to 3 different isoforms have been reported for this protein. It is reported to be expressed in the hippocampus, nucleus raphe, and cortex. A member of the Peptidase M14 protein family, CPA6 is suspected to be involved in the proteolytic inactivation of enkephalins and neurotensin in some brain areas. Post-translational modifications have been described, including glycosylation.
The CPA6 marker can be used to identify Midbrain Splatter Neurons.* Synonyms for this target antigen include carboxypeptidase B. CPA6 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Western Blot is the most common application for the CPA6 antibodies listed below. ELISA and Immunohistochemistry are also common applications.
*HuBMAP Human Reference Atlas v1.4