Anti-ANO4 antibodies are used in the immunodetection of the protein anoctamin 4. In humans, the canonical protein has a reported length of 955 amino acid residues and a mass of 111.5 kDa. Its subcellular localization is in the cell membrane. Up to 3 different isoforms have been reported for this protein. A member of the Anoctamin protein family, ANO4 is known to have calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (By similarity). Post-translational modifications have been described, including glycosylation. Synonyms for this target antigen include transmembrane protein 16D. ANO4 gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species. ELISA is a widely used application for the ANO4 antibodies listed below. In addition, Western Blot is also a common application.