Anti-ubiquitin B antibodies are used for the immunodetection of the protein encoded by the UBB gene. In humans, the canonical protein has a reported length of 229 amino acid residues and a mass of 25.8 kDa. Its subcellular localization is in the membrane, nucleus, mitochondria, and cytoplasm. A member of the Ubiquitin protein family, it is known to be involved with protein ubiquitination. Post-translational modifications have been described, including ubiquitination and phosphorylation. Other names for this target antigen include polyubiquitin-B, epididymis secretory protein Li 50, polyubiquitin B, and HEL-S-50. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 2500 citations in the literature describe the use of ubiquitin B antibodies in research. Western Blot is a widely used application for these antibodies. ELISA, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry are also common applications.