Anti-RPUSD4 antibodies are used in the immunodetection of the protein RNA pseudouridine synthase D4. In humans, the canonical protein has a reported length of 377 amino acid residues and a mass of 42.2 kDa. Its subcellular localization is in the nucleus, mitochondria, and cytoplasm. Up to 2 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. A member of the Pseudouridine synthase RluA protein family, RPUSD4 is known to catalyze uridine to pseudouridine isomerization (pseudouridylation) of different mitochondrial RNA substrates. Synonyms for this target antigen include RNA pseudouridylate synthase domain containing 4, RNA pseudouridylate synthase domain-containing protein 4, mitochondrial RNA pseudouridine synthase RPUSD4, and pseudouridylate synthase RPUSD4, mitochondrial. RPUSD4 gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species. Some RPUSD4 antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.