Anti-LGMN antibodies are used in the immunodetection of the protein legumain. In humans, the canonical protein has a reported length of 433 amino acid residues and a mass of 49.4 kDa. Its subcellular localization is in the lysosomes. Up to 3 different isoforms have been reported for this protein. It is ubiquitously expressed across many tissue types. A member of the Peptidase C13 protein family, LGMN is known to have a strict specificity for hydrolysis of asparaginyl bonds. Post-translational modifications have been described, including glycosylation.
The LGMN marker can be used to identify Thymic Macrophages, Thymic Cortical Macrophages, and Thymic Medullary Macrophages.* Synonyms for this target antigen include LGMN1, PRSC1, asparaginyl endopeptidase, cysteine protease 1, protease, cysteine 1, and AEP. LGMN gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 60 citations in the literature describe the use of LGMN antibodies in research. Western Blot is a widely used application for these antibodies. In addition, ELISA is also a common application.
*HuBMAP Human Reference Atlas v1.4