Anti-centromere protein N antibodies are used in the immunodetection of the protein encoded by the CENPN gene. In humans, the canonical protein has a reported length of 339 amino acid residues and a mass of 39.6 kDa. Its subcellular localization is in the nucleus. Alternative splicing is reported to yield 5 different isoforms for this protein. A member of the CENP-N/CHL4 protein family, it is a known component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. Other names for this target antigen include interphase centromere complex protein 32.