Anti-glyceraldehyde-3-phosphate dehydrogenase antibodies are used in the immunodetection of the protein encoded by the GAPDH gene. In humans, the canonical protein has a reported length of 335 amino acid residues and a mass of 36.1 kDa. Its subcellular localization is in the nucleus and cytoplasm. Alternative splicing is reported to yield 2 different isoforms for this protein. A member of the Glyceraldehyde-3-phosphate dehydrogenase protein family, it is known to have both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Post-translational modifications have been described, including glycosylation, ubiquitination and phosphorylation. Other names for this target antigen include GAPD, HEL-S-162eP, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, and G3PD.