Anti-ACTN4 antibodies are used in the immunodetection of the protein actinin alpha 4. In humans, the canonical protein has a reported length of 911 amino acid residues and a mass of 104.9 kDa. Its subcellular localization is in the nucleus and cytoplasm. Up to 3 different isoforms have been reported for this protein. It is widely expressed across many tissue types. A member of the Alpha-actinin protein family, ACTN4 is known to be involved with cytoskeleton organization and the regulation of cell migration. The ACTN4 gene has been associated with the disease, Focal segmental glomerulosclerosis. Synonyms for this target antigen include FSGS, FSGS1, alpha-actinin-4, focal segmental glomerulosclerosis 1, non-muscle alpha-actinin 4, and ACTININ-4. ACTN4 gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species. Over 220 citations in the literature describe the use of ACTN4 antibodies in research. Western Blot is a widely used application for these antibodies. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.