Anti-NGB antibodies are used in the immunodetection of the protein neuroglobin. In humans, the canonical protein has a reported length of 151 amino acid residues and a mass of 16.9 kDa. Its subcellular localization is in the mitochondria and cytoplasm. It is reported to be highly expressed in the brain, the strongest expression is seen in the frontal lobe, the subthalamic nucleus and the thalamus. A member of the Globin protein family, NGB is known to be involved in oxygen transport in the brain. Post-translational modifications have been described, including phosphorylation. NGB gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of NGB antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.