Anti-ENPEP antibodies are used in the immunodetection of the protein glutamyl aminopeptidase. In humans, the canonical protein has a reported length of 957 amino acid residues and a mass of 109.2 kDa. Its subcellular localization is in the cell membrane. It is reported to be expressed in choriocarcinoma cancer cell lines. A member of the Peptidase M1 protein family, Enpep is reported to regulate central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II. Post-translational modifications have been described, including glycosylation. Synonyms for this target antigen include CD249, gp160, AP-A, EAP, aminopeptidase A, differentiation antigen gp160, and APA. ENPEP gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 210 citations in the literature describe the use of Enpep antibodies in research. Immunohistochemistry is a widely used application for these antibodies. Flow Cytometry and Western Blot are also common applications.