Anti-RNA pseudouridine synthase D4 antibodies are used for the immunodetection of the protein encoded by the RPUSD4 gene. In humans, the canonical protein has a reported length of 377 amino acid residues and a mass of 42.2 kDa. Its subcellular localization is in the nucleus, mitochondria, and cytoplasm. Alternative splicing is reported to yield 2 different isoforms for this protein. A member of the Pseudouridine synthase RluA protein family, it is known to catalyze uridine to pseudouridine isomerization (pseudouridylation) of different mitochondrial RNA substrates. Other names for this target antigen include RNA pseudouridylate synthase domain containing 4, RNA pseudouridylate synthase domain-containing protein 4, mitochondrial RNA pseudouridine synthase RPUSD4, and pseudouridylate synthase RPUSD4, mitochondrial. Gene orthologs have been identified in the mouse, rat, bovine, frog, chimpanzee and chicken species.