Anti-asparaginyl-tRNA synthetase 1 antibodies are used for the immunodetection of the protein encoded by the NARS1 gene. In humans, the canonical protein has a reported length of 548 amino acid residues and a mass of 62.9 kDa. Its subcellular localization is in the cytoplasm. Alternative splicing is reported to yield 2 different isoforms for this protein. A member of the Class-II aminoacyl-tRNA synthetase protein family, it is known to catalyze the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn). The gene encoding this protein is implicated in Neurodevelopmental disorder with microcephaly, impaired language, and gait abnormalities. Other names for this target antigen include NARS, NEDMILEG, NEDMILG, asparagine--tRNA ligase, cytoplasmic, asparagine tRNA ligase 1, cytoplasmic, and ASNRS. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.