Anti-T-box transcription factor 5 antibodies are used for the immunodetection of the protein encoded by the TBX5 gene. In humans, the canonical protein has a reported length of 518 amino acid residues and a mass of 57.7 kDa. Its subcellular localization is in the nucleus and cytoplasm. Alternative splicing is reported to yield 3 different isoforms for this protein. It is reported to be a dna-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation. Post-translational modifications have been described, including acetylation. The gene encoding this protein is implicated in Holt-Oram syndrome. Other names for this target antigen include T-box transcription factor TBX5, T-box 5, T-box protein 5, and HOS. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.