Anti-PKM antibodies are used in the immunodetection of the protein pyruvate kinase M1/2. In humans, the canonical protein has a reported length of 531 amino acid residues and a mass of 57.9 kDa. Its subcellular localization is in the nucleus and cytoplasm. Up to 3 different isoforms have been reported for this protein. A member of the Pyruvate kinase protein family, PKM is known to catalyze the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Post-translational modifications have been described, including acetylation and phosphorylation. PKM is also used as a cellular marker for characterizing astrocytes. Synonyms for this target antigen include HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, p58, and CTHBP. PKM gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species.