Anti-ATP5F1E antibodies are used in the immunodetection of the protein ATP synthase F1 subunit epsilon. In humans, the canonical protein has a reported length of 51 amino acid residues and a mass of 5.8 kDa. Its subcellular localization is in the mitochondria. It is ubiquitously expressed across many tissue types. It is a member of the Eukaryotic ATPase epsilon protein family. The ATP5F1E gene has been associated with the disease, Mitochondrial complex V deficiency. Synonyms for this target antigen include ATPE, MC5DN3, ATP synthase subunit epsilon, mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit, and ATP5E. ATP5F1E gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Some ATP5F1E antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.