Anti-RUBCNL antibodies are used in the immunodetection of the protein rubicon like autophagy enhancer. In humans, the canonical protein has a reported length of 662 amino acid residues and a mass of 73.5 kDa. Its subcellular localization is in the cytoplasmic vesicles and membrane. Up to 6 different isoforms have been reported for this protein. It is noted to be expressed weakly in cervical carcinoma cell lines. RUBCNL is a reported regulator of autophagy that promotes autophagosome maturation by facilitating the biogenesis of phosphatidylinositol 3-phosphate (PtdIns(3)P) in late steps of autophagy. Synonyms for this target antigen include KIAA0226 like, RUN and cysteine rich domain containing beclin 1 interacting protein like, protein RUBCNL-like, protein Rubicon-like, and protein associated with UVRAG as autophagy enhancer. RUBCNL gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Some RUBCNL antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. Immunocytochemistry and Immunofluorescence are also common applications.