Anti-TWNK antibodies are used in the immunodetection of the protein twinkle mtDNA helicase. In humans, the canonical protein has a reported length of 684 amino acid residues and a mass of 77.2 kDa. Its subcellular localization is in the mitochondria. Up to 3 different isoforms have been reported for this protein. TWNK is known to be a mitochondrial helicase involved in mtDNA replication and repair. Synonyms for this target antigen include T7 helicase-related protein with intramitochondrial nucleoid localization, T7-like mitochondrial DNA helicase, progressive external ophthalmoplegia 1 protein, and T7 gp4-like protein with intramitochondrial nucleoid localization. TWNK gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of TWNK antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.