Anti-BRF1 antibodies are used in the immunodetection of the protein BRF1 RNA polymerase III transcription initiation factor subunit. In humans, the canonical protein has a reported length of 677 amino acid residues and a mass of 73.8 kDa. Its subcellular localization is in the nucleus. Up to 9 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. A member of the TFIIB protein family, BRF1 is reported to be a general activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. Synonyms for this target antigen include CFDS, GTF3B, HEL-S-76p, TAF3B2, TAF3C, TAFIII90, TF3B90, and BRF. BRF1 gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species. A number of BRF1 antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.