Anti-AFDN antibodies are used in the immunodetection of the protein afadin, adherens junction formation factor. In humans, the canonical protein has a reported length of 1824 amino acid residues and a mass of 206.8 kDa. Its subcellular localization is in the cell junctions. Up to 6 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. AFDN is known to be involved with cell adhesion and cell to cell signaling. Synonyms for this target antigen include MLL-AF6, MLLT4, l-afadin, afadin, and AF6. AFDN gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 80 citations in the literature describe the use of AFDN antibodies in research. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.