Anti-LIM domain kinase 1 antibodies are used in the immunodetection of the protein encoded by the LIMK1 gene. In humans, the canonical protein has a reported length of 647 amino acid residues and a mass of 72.6 kDa. Its subcellular localization is in the nucleus and cytoplasm. Alternative splicing is reported to yield 4 different isoforms for this protein. It is reported be highly expressed in both adult and fetal nervous system. A member of the TKL Ser/Thr protein kinase protein family, it is a type of serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Post-translational modifications have been described, including ubiquitination and phosphorylation. Other names for this target antigen include LIMK and LIM motif-containing protein kinase.