Anti-DEAD-box helicase 25 antibodies are used for the immunodetection of the protein encoded by the DDX25 gene. In humans, the canonical protein has a reported length of 483 amino acid residues and a mass of 54.7 kDa. Its subcellular localization is in the nucleus and cytoplasm. It is reported to be highly expressed in the Leydig and germ cells of the testis and weakly expressed in the pituitary and hypothalamus. A member of the DEAD box helicase protein family, it is known to be involved with spermatid development. Post-translational modifications have been described, including phosphorylation. Other names for this target antigen include ATP-dependent RNA helicase DDX25, DEAD (Asp-Glu-Ala-Asp) box helicase 25, DEAD (Asp-Glu-Ala-Asp) box polypeptide 25, DEAD box protein 25, and GRTH. Gene orthologs have been identified in the mouse, rat, bovine, frog and chimpanzee species. These DEAD-box helicase 25 antibodies are widely used in Western Blot experiments, among other applications.