Anti-glycoprotein nmb antibodies are used for the immunodetection of the protein encoded by the GPNMB gene. In humans, the canonical protein has a reported length of 572 amino acid residues and a mass of 63.9 kDa. Its subcellular localization is in the cell membrane. Alternative splicing is reported to yield 2 different isoforms for this protein. It is reported to be widely expressed, but very low expression, if any, in the brain. A member of the PMEL/NMB protein family, it is suspected to be a melanogenic enzyme. Post-translational modifications have been described, including glycosylation.
The glycoprotein nmb marker can be used to characterize Choroid Plexus Non-Neuronal Cells.* Other names for this target antigen include NMB, PLCA3, transmembrane glycoprotein NMB, glycoprotein (transmembrane) nmb, and HGFIN. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 90 citations in the literature describe the use of glycoprotein nmb antibodies in research. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.
*HuBMAP Human Reference Atlas v1.4