Anti-CHRNA5 antibodies are used in the immunodetection of the protein cholinergic receptor nicotinic alpha 5 subunit. In humans, the canonical protein has a reported length of 468 amino acid residues and a mass of 53.1 kDa. Its subcellular localization is in the cell membrane. It is notably expressed in the smooth muscle, hippocampus, and cerebral cortex. A member of the Ligand-gated ion channel (TC 1.A.9) protein family, CHRNA5 is known to be involved with chemical synaptic transmission and signal transduction pathways. Synonyms for this target antigen include neuronal acetylcholine receptor subunit alpha-5, Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5, acetylcholine receptor, nicotinic, alpha 5 (neuronal), and LNCR2. CHRNA5 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of CHRNA5 antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. In addition, ELISA is also a common application.