Anti-DEAD-box helicase 28 antibodies are used in the immunodetection of the protein encoded by the DDX28 gene. In humans, the canonical protein has a reported length of 540 amino acid residues and a mass of 59.6 kDa. Its subcellular localization is in the nucleus and mitochondria. It is reported to be expressed in all tissues tested, including brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, leukocytes, colon, small intestine, ovary and prostate. A member of the DEAD box helicase protein family, it is known to play an important role in facilitating the proper assembly of the mitochondrial large ribosomal subunit and its helicase activity is essential for this function. Other names for this target antigen include DEAD (Asp-Glu-Ala-Asp) box polypeptide 28, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 28, mitochondrial DEAD box protein 28, and probable ATP-dependent RNA helicase DDX28.