Anti-BUB3 antibodies are used in the immunodetection of the protein BUB3 mitotic checkpoint protein. In humans, the canonical protein has a reported length of 328 amino acid residues and a mass of 37.2 kDa. Its subcellular localization is in the nucleus. Up to 2 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. A member of the WD repeat BUB3 protein family, BUB3 is known to have a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Synonyms for this target antigen include hBUB3, mitotic checkpoint protein BUB3, BUB3 budding uninhibited by benzimidazoles 3 homolog, budding uninhibited by benomyl, and BUB3L. BUB3 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of BUB3 antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.