Anti-NCL antibodies are used in the immunodetection of the protein nucleolin. In humans, the canonical protein has a reported length of 710 amino acid residues and a mass of 76.6 kDa. Its subcellular localization is in the nucleus and cytoplasm. It is notably widely expressed in many tissue types. NCL is known to be involved with angiogenesis and the regulation of transcription. Post-translational modifications have been described, including methylation. Synonyms for this target antigen include C23 and Nsr1. NCL gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 360 citations in the literature describe the use of NCL antibodies in research. Flow Cytometry, Western Blot, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry are common applications for these antibodies.