Anti-ANKRD53 antibodies are used in the immunodetection of the protein ankyrin repeat domain 53. In humans, the canonical protein has a reported length of 530 amino acid residues and a mass of 59.6 kDa. Its subcellular localization is in the cytoplasm. Up to 2 different isoforms have been reported for this protein. It is notably expressed in soft tissue and testis. ANKRD53 is reported to be required for normal progression through mitosis. Post-translational modifications have been described, including phosphorylation. Synonyms for this target antigen include ankyrin repeat domain-containing protein 53. ANKRD53 gene orthologs have been reported in the mouse, rat, bovine, frog and chimpanzee species. Some ANKRD53 antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. Flow Cytometry, Immunofluorescence, and Immunohistochemistry are also common applications.