Anti-PNKP antibodies are used in the immunodetection of the protein polynucleotide kinase 3'-phosphatase. In humans, the canonical protein has a reported length of 521 amino acid residues and a mass of 57.1 kDa. Its subcellular localization is in the nucleus and chromosomes. Up to 2 different isoforms have been reported for this protein. It is reported to be expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine. A member of the DNA 3' phosphatase protein family, PNKP is known to play an important role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Synonyms for this target antigen include CMT2B2, EIEE10, MCSZ, PNK, bifunctional polynucleotide phosphatase/kinase, and AOA4. PNKP gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish and chimpanzee species. A number of PNKP antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.