Bst Polymerase, or Bst DNA Polymerase I, was isolated from Bacillus stearothermophilus. This 97 kDa enzyme features a similar 5’-3’ polymerase activity of E. coli, but lacks the 3’-5’ exonuclease activity. Bst polymerase is recommended for applications such as Isothermal amplification (LAMP) and DNA sequencing through high GC regions.