Western blot was first introduced by Towbin, et al. in 1979 and has since become a common laboratory technique. Near-infrared fluorescence detection on the Odyssey Classic, Odyssey CLx, Odyssey Fc, or Odyssey Sa Imaging Systems provides a quantitative two-color detection method for Western blots. In this guide, read some background on how to use NIR for Western blot, important factors for correct detection and tips for optimizing image capture and data analysis.
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