HRP Substrates

Horseradish peroxidase (HRP) substrates are used in the immunostaining and immunodetection of specific proteins and antigens. HRP is a widely used label, often conjugated to secondary antibodies in immunochemical applications such as immunohistochemistry, western blotting, and ELISA. Common HRP substrates include: ABTS, AEC, DAB, ECL, TMB, and Amplex Red. When choosing a HRP substrate, consider the desired colors manifested in the final staining to ensure proper differentiation.

HRP Substrates

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MyBioSource.com

3,3',5,5'-Tetramethylbenzidine (TMB) Substrate (1-Component) for ELISA

3,3',5,5'-Tetramethylbenzidine (TMB) Substrate (1-Component) for ELISA
  • Quantity:
    100 mL
  • Applications:
    ELISA
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
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BPS Bioscience, Inc.

ELISA ECL Substrate

ELISA ECL Substrate
  • Quantity:
    500 ml
  • Applications:
    EIA, Immunoassay, ELISA
  • Detection Method:
    Chemiluminescent, Luminescent
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BioLegend

AEC Chromogen Concentrate

AEC Chromogen Concentrate
  • Quantity:
    1 mL
  • Applications:
    Immunohistochemistry
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
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Rockland Immunochemicals, Inc.

TMB ELISA PEROXIDASE SUBSTRATE

TMB ELISA PEROXIDASE SUBSTRATE
  • Quantity:
    1 L
  • Applications:
    EIA, Immunoassay, ELISA
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Citations:
(22)
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Rockland Immunochemicals, Inc.

Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate

Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate
  • Quantity:
    110 mL
  • Applications:
    EIA, Immunoassay, ELISA, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Citations:
(27)
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MyBioSource.com

TMB for Histochemistry

TMB for Histochemistry
  • Quantity:
    500 ml
  • Applications:
    ELISA
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
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AEC Chromogen Kit

AEC Chromogen Kit
  • Quantity:
    1 Kit
  • Applications:
    Immunohistochemistry
  • Detection Method:
    Colorimetric
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Advansta

WesternBright Quantum HRP Substrate

WesternBright Quantum HRP Substrate
  • Quantity:
    100ml
  • Applications:
    EIA, Immunoassay, Labeling, Staining, Blotting, ELISA, Western Blot
  • Detection Method:
    Chemiluminescent, Colorimetric
  • Format:
    Liquid
Citations:
(21)
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WesternBright ECL Spray-HRP Substrate

WesternBright ECL Spray-HRP Substrate
  • Quantity:
    500ml
  • Applications:
    Western Blot
  • Detection Method:
    Chemiluminescent
  • Format:
    Liquid
Citations:
(21)
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BioLegend

AEC Substrate Buffer

AEC Substrate Buffer
  • Quantity:
    50 mL
  • Applications:
    Immunohistochemistry
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
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Stop Solution for TMB Substrate

Stop Solution for TMB Substrate
  • Quantity:
    60 mL
  • Applications:
    EIA, Immunoassay, ELISA
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Citations:
(24)
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BPS Bioscience, Inc.

ELISA ECL Substrate

ELISA ECL Substrate
  • Quantity:
    200 ml
  • Applications:
    EIA, Immunoassay, ELISA
  • Detection Method:
    Chemiluminescent, Luminescent
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ELISA ECL Substrate 1L

ELISA ECL Substrate 1L
  • Quantity:
    1 L
  • Applications:
    EIA, Immunoassay, ELISA
  • Detection Method:
    Chemiluminescent, Luminescent
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Bio-Rad

Clarity Max™ Western ECL Substrate, 100 ml

Clarity Max™ Western ECL Substrate, 100 ml
  • Quantity:
    Inquire
  • Applications:
    Western Blot
  • Detection Method:
    Chemiluminescent
  • Format:
    Liquid
Citations:
(224)
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Clarity Western ECL Substrate, 500 ml

Clarity Western ECL Substrate, 500 ml
  • Quantity:
    Inquire
  • Applications:
    Western Blot
  • Detection Method:
    Chemiluminescent
  • Format:
    Liquid
Citations:
(683)
Figures:
(1)
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Promega

ECL Western Blotting Substrate

ECL Western Blotting Substrate
  • Quantity:
    250ml
  • Applications:
    Western Blot
  • Detection Method:
    Chemiluminescent
  • Format:
    Liquid
Citations:
(56)
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ECL Western Blotting Substrate

ECL Western Blotting Substrate
  • Quantity:
    500ml
  • Applications:
    Western Blot
  • Detection Method:
    Chemiluminescent
  • Format:
    Liquid
Citations:
(56)
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R&D Systems, a Bio-Techne Brand

DAB Enhancer

DAB Enhancer
  • Quantity:
    1 mL
  • Applications:
    Immunohistochemistry
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
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Novus Biologicals, a Bio-Techne Brand

AEC Chromogen/Substrate

AEC Chromogen/Substrate
  • Quantity:
    30 ml
  • Applications:
    Immunohistochemistry, Immunohistochemistry - fixed, Immunohistochemistry - frozen
  • Detection Method:
    Colorimetric
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ImmPACT (TM) DAB EqV HRP Substrate

ImmPACT (TM) DAB EqV HRP Substrate
  • Quantity:
    400 ml (also 100 ml)
  • Applications:
    In Situ Hybridization, Dot Blot, ELISPOT, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry - fixed, Immunohistochemistry - frozen, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
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Abcam

450 nm Stop Solution for TMB Substrate

450 nm Stop Solution for TMB Substrate
  • Quantity:
    100 ML, 1000 ML
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Citations:
(12)
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650 nm Stop Solution for TMB Substrate

650 nm Stop Solution for TMB Substrate
  • Quantity:
    100 ML, 1000 ML
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Citations:
(1)
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Showing 10 of 18 suppliers (156 products total)

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Bio-Rad
Clarity Western ECL Substrate, 500 ml
1705061
1
1 Figure from 683 Publications
Bio-Rad
Clarity™ and Clarity Max™ Western ECL Blotting Substrates
1705060S / 1705060 / 1705061 / 1705062S / 1705062 | EA
1
1 Figure from 1440 Publications
Showing 2 of 2 products
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