Poor Signal with ROR Gamma (t) Antibody, No Distinct Positive Population

March 31, 2023

BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific (eBioscience)

Product Name:

PE-ROR gamma (t) Monoclonal Antibody (AFKJS-9)

Catalog Number:

12-6988-82

Clone Number:

N/A

I'm interested in studying how diets and dietary metabolites affect host immune response, with a focus of CD4 T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse TH17 cells

Primary Incubation

1:20

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

Splenic CD4 T cells were polarized toward TH17 conditions. Cells were stained with the anti-Rorgt antibody but did not see a clear distinct positive population.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

N/A

The Bad

Expensive and no clear signal.

The Bottom Line

Not recommended.

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