Very Specific Signal with EMP/MAEA Antibody

University of Cambridge

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Human/Mouse/Rat EMP/MAEA Antibody

Catalog Number:

AF7288

Clone Number:

Polyclonal

Info:Supplier Page View Product Specs

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HEK293 cells were lysed with RIPA buffer before spinning down, mixed with loading buffer and run on a 12% Gel, and transferred to PVDF membrane in 5% methanol.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293

Primary Incubation

1 µg/mL

Blocking Agent

5% BSA

Secondary Incubation

1:3000 anti-SheepHRP

Tertiary Incubation

NA

Detection

ECL

Results Summary

Lysates from HEK293 cells were run on a 12% and transfered onto a PVDF membrane. Membrane was probed with 1 µg/mL anti-MAEA overnight washing and incubating with secondary.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good signal.

The Bad

Nothing of note.

The Bottom Line

Good antibody.

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