Bright CD62P Staining on Human Platelets

Hem/Onc
Boston Children's Hospital
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

BV480 Mouse Anti-Human CD62P

Catalog Number:

746444

Clone Number:

AK-4

Info:Supplier Page 

Browse Similar Products: CD62P Antibodies

CD62P is present in the granules of resting platelets and expressed on the cell surface following platelet activation. BV480 Mouse Anti-Human CD62P allows the clear resolution of activated/degranulated platelets from resting/nondegranulated platelets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human whole blood collected in sodium citrate

Primary Incubation

30 min at room temperature

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Citrate-anticoagulated human whole blood was diluted 1:10 in HEPES-Tyrodes-BSA containing BV480 Mouse Anti-Human CD62P with or without 10 µg/mL CRP-XL (platelet agonist). Lyse/fix buffer was added, and samples were acquired by flow cytometry. BV480 Mouse Anti-Human CD62P allowed clear resolution of activated/degranulated platelets.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright CD62P staining.

The Bad

N/A

The Bottom Line

BV480 Mouse Anti-Human CD62P allows good detection of CD62P on the surface of activated/degranulated platelets.

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