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Brilliant Violet 421™ anti-mouse F4/80 Antibody
123137
BM8
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I am interested to study myeloid cells in steady state and inflammation. As a positive control for F4/80 I stained the small intestine.
Immunofluorescence
Cryosection of murine small intestine
1:100 overnight at RT
1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum
N/A
Image was acquired with Zeiss LSM780 confocal microscope.
Excellent staining of F480+ Macrophages (red) in the lamina propria of the small intestine, Actin (Phalloidin) in green.
Very good staining of macrophages.
None
Highly recommended to stain Macrophages in mouse crysections.