Great Isotype Control

Immunology
UiO
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Catalog Number:

17-4714-82

Clone Number:

N/A

Our research is heavily relied on cell-based assays. We often need to characterize cell lines or primary cells, using FACs, which requires the usage of isotype controls.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Cell lines

Primary Incubation

1:100, 15min RT

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

An EBV-immortalized cell line was generated from blood donor PBMCs. We are interested in sorting the IGA positive B-cells, thus an a-IGHA antibody(APC) was used. The equivalent isotype control was used to separate background noise from stained cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use, works wells

The Bad

None

The Bottom Line

Good isotype control that requires low volumes of the antibody.

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