R&D Systems
Mouse TNF-alpha DuoSet ELISA
DY410
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Used to study the Zymosan induced TNFa response from isolated mouse neutrophils. Saw robust and reproducible response across many experiments using this kit.
ELISA
Mouse neutrophils
Used as per instructions
Very reproducible, the figure below shows the TNFa released into the supernatant from isolated mouse neutrophils, stimulated for 4 hours with opsonised zymosan
N/A
Very reliable.
Nothing
Definitely worth the money, easy to handle and optimize.