PROGEN's Anti-Perilipin 2 Polyclonal Serum for Staining Lipid Droplet Proteins

October 03, 2022

Albert Einstein College of Medicine

Overall

Quality of Results

Ease-of-Optimization

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Company:

PROGEN

Product Name:

anti-Perilipin 2 guinea pig polyclonal serum

Catalog Number:

GP40

Clone Number:

N/A (polyclonal)

Our laboratory stains for proteins associated with lipid droplets within brain sections isolated from our murine models of neurodegeneration and aging using this polyclonal serum from PROGEN as outlined here.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain sections

Primary Incubation

1:200 O/N @ 4 degrees C

Blocking Agent

BSA/Serum

Secondary Incubation

Alexa conjugated rabbit anti-guinea pig 1:500 for 1 hour @ RT

Tertiary Incubation

N/A

Detection

Fluorescent or confocal microscopy

Results Summary

The antibody provides clear staining of the lipid droplet-associated protein perilipin 2 once optimized (or used as described here).

DOI or PMID #

N/A

Additional Notes

Diffuse background staining is normal / to be expected alongside the specific staining.

Image Gallery

Summary

The Good

Provides reliable and repeatable staining results; Easy to use / optimize.

The Bad

Mouse brain tissue exhibits diffuse background staining when used with this reagent.

The Bottom Line

We recommend this product for the detection of lipid droplets in murine brain tissue sections, specifically those isolated from murine models of neurodegeneration and aging.

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