R&D's Polyclonal Anti-Mouse PDGF R Alpha Stains Immature Oligodendrocytes

September 30, 2022

Albert Einstein College of Medicine

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

R&D Systems

Product Name:

Mouse PDGF R alpha Antibody

Catalog Number:

AF1062

Clone Number:

N/A (polyclonal)

We identify and characterize mature oligodendrocytes in mouse brain sections from murine models of neurodegeneration and aging using this polyclonal antibody reagent from R&D Systems.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain sections

Primary Incubation

1:500 O/N @ 4 degrees C

Blocking Agent

BSA/Serum

Secondary Incubation

Alexa conjugated donkey anti-goat; 1:500 for 1 hour @ RT

Tertiary Incubation

N/A

Detection

Fluorescent or confocal microscopy

Results Summary

This antibody must be tested and optimized with new workflows, or you can expect background staining to be present. Reducing background staining is important for clear imaging to ensure reliable and repeatable results.

DOI or PMID #

N/A

Additional Notes

Overexposure is required to ensure visible staining.

Image Gallery

Summary

The Good

The staining provides a clear picture of the distribution of immature oligodendrocytes throughout the brain section/tissues under review.

The Bad

Under certain conditions, the antibody may need to be used a higher concentration, though this can be readily addressed during optimization stages.

The Bottom Line

We recommend this product for use as described here to stain immature oligodendrocytes in murine models of neurodegeneration and aging.

Share your experience with other scientists. Write a Review! »

Join the discussion