Anti-Doublecortin Polyclonal Antibody Detects Doublecortin Positive Immature Neurons

September 30, 2022

Albert Einstein College of Medicine

Overall

Quality of Results

Ease-of-Optimization

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Company:

Millipore Sigma

Product Name:

Anti-Doublecortin Antibody

Catalog Number:

AB2253

Clone Number:

N/A (polyclonal)

Info:Supplier Page 

Browse Similar Products: Doublecortin Antibodies

Our laboratory employ's Millipore Sigma's polyclonal anti-Doublecortin antibody to stain mouse brain tissues for Doublecortin positive immature neurons, specifically within neurodegenerative niches of the brain.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain sections

Primary Incubation

1:500 O/N @ 4 degrees C

Blocking Agent

BSA/Serum

Secondary Incubation

Alexa conjugated goat anti-guinea pig; 1:500 for 1 hour @ RT

Tertiary Incubation

N/A

Detection

Fluorescent or confocal microscopy

Results Summary

The antibody provides clear staining of immature neurons in the subgranular zone and subventricular zone of mouse brain once optimized (or used as described here).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Neurons at every stage of development can be detected using this reagent; Once optimized, the results are robust and repeatable.

The Bad

The stain does not detect specific stages of early neuron development, so additional antibodies may be needed for certain workflows; Requires optimization for use and mitigation of overexposure.

The Bottom Line

The bottom line: When used as described, we recommend this product for staining of neurons at every stage of early development.

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