Cell Signaling Technology
Anti-CD45 antibody
70257
N/A
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Mouse heart was fixed in 4% paraformaldehyde. The fixed heart tissues were dehydrated and embedded in paraffin. For immunofluorescence staining, heart sections were incubated with anti-CD45 and anti-F4/80 antibody (1:100, Cell Signaling Technology, USA) at 4◦C overnight. After 3 times wash with PBS, fluorescein-isothiocyanate-conjugated secondary antibodies (1:1,000, Cell Signaling Technology, USA) were incubated at room temperature in the dark for 1 h. After DAPI (Sigma-Aldrich, USA) staining, fluorescence images were captured under the fluorescence microscope (Leica, Wetzlar, Germany). Representative immunofluorescence images showed the CD45+ inflammatory cells and CD45+F4/80+ macrophages in the heart.
Immunofluorescence
Mouse heart
1/100 dilution 4°C overnight incubation
5% BSA
1/1000 dilution room temperature 1 h incubation
Room temperature incubation with DAPI
Fluorescence microscope (Leica, Wetzlar, Germany)
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FIGURE 2m
The antibody works well as expected.
The antibody works.