Abcam
Anti-CD63 antibody
ab217345
N/A
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Isolation of murine cardiac extracellular vesicles (EVs) was performed using differential centrifugation. Protein immunoblots of four typical exosomal markers (Alix, Tsg101, CD63 and CD9) for EVs, including increased release of EVs (IR-EVs) and EVs from the sham heart (S-EVs).
Western Blot
Murine EVs
1/500 dilution 4°C overnight incubation
5% milk
1/2000 dilution room temperature 1 h incubation
Room temperature 10 min incubation
ECL
33664937
Fig. 1b
The antibody works well as expected.
The antibody works.