Very Good Anti-CD9 Antibody

September 27, 2022

pathology
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-CD9 antibody

Catalog Number:

ab223052

Clone Number:

N/A

Info:Supplier Page View Product Specs

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Isolation of murine cardiac extracellular vesicles (EVs) was performed using differential centrifugation. Protein immunoblots of four typical exosomal markers (Alix, Tsg101, CD63 and CD9) for EVs, including increased release of EVs (IR-EVs) and EVs from the sham heart (S-EVs).

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine EVs

Primary Incubation

1/1000 dilution 4°C overnight incubation

Blocking Agent

5% milk

Secondary Incubation

1/2000 dilution room temperature 1 h incubation

Tertiary Incubation

room temperature 10 min incubation

Detection

ECL

Results Summary

Isolation of murine cardiac extracellular vesicles (EVs) was performed using differential centrifugation. Protein immunoblots of four typical exosomal markers (Alix, Tsg101, CD63 and CD9) for EVs, including increased release of EVs (IR-EVs) and EVs from the sham heart (S-EVs).

DOI or PMID #

33664937

Additional Notes

Fig. 1b

Image Gallery

Summary

The Good

The antibody works well as expected.

The Bad

N/A

The Bottom Line

The antibody works well.

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