Anti-USP47 Antibody Works Well

September 26, 2022

pathology
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

GeneTex

Product Name:

Anti-USP47 antibody

Catalog Number:

GTX106253

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: USP47 Antibodies

HEK293T cells stably expressing control (Ctrl), USP47shRNA, USP20 shRNA or a combination of USP20 and USP47 shRNAs were treated with MG132 for 6 h before harvest. β-catenin was immunoprecipitated and blots probed with the indicated antibodies. The results indicated that depletion of USP20 or USP47 in HEK293T cells increased the polyubiquitination of β-catenin.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293 whole cell lysates

Primary Incubation

1/1000 dilution 4°C overnight incubation

Blocking Agent

5% milk

Secondary Incubation

1/2000 dilution room temperature 1 h incubation

Tertiary Incubation

Room temperature 10 min incubation

Detection

ECL

Results Summary

HEK293T cells stably expressing control (Ctrl), USP47shRNA, USP20 shRNA or a combination of USP20 and USP47 shRNAs were treated with MG132 for 6 h before harvest. β-catenin was immunoprecipitated and blots probed with the indicated antibodies. The results indicated that depletion of USP20 or USP47 in HEK293T cells increased the polyubiquitination of β-catenin.

DOI or PMID #

https://static-content.springer.com/esm/art%3A10.1038%2Fs41418-018-0138-z/MediaObjects/41418_2018_138_MOESM1_ESM.pdf

Additional Notes

Figure S4A

Image Gallery

Summary

The Good

The antibody works well as expected.

The Bad

N/A

The Bottom Line

The antibody works well.

Share your experience with other scientists. Write a Review! »

Join the discussion