Great Anti-His Antibody

September 23, 2022

pathology
University of Pittsburgh
Research Scientist

Overall

Quality of Results

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Company:

Santa Cruz Biotechnology

Product Name:

Anti-His antibody

Catalog Number:

sc-8036

Clone Number:

N/A

Info:Supplier Page View Product Specs

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HEK293T cells stably expressing control (Ctrl) or USP20 shRNAs were transfected with indicated constructs. After 48 h, cells were treated with MG132 for 6 h before collecting and Ni-NTA beads were used to pull down His-tagged ubiquitin. Blots were probed with the indicated antibodies. The results indicated that USP20 deubiquitinated β-catenin.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293 whole cell lysates

Primary Incubation

1/1000 dilution 4°C overnight incubation

Blocking Agent

5% milk

Secondary Incubation

1/2000 dilution room temperature 1 h incubation

Tertiary Incubation

Room temperature 10 min incubation

Detection

ECL

Results Summary

HEK293T cells stably expressing control (Ctrl) or USP20 shRNAs were transfected with indicated constructs. After 48 h, cells were treated with MG132 for 6 h before collecting and Ni-NTA beads were used to pull down His-tagged ubiquitin. Blots were probed with the indicated antibodies. The results indicated that USP20 deubiquitinated β-catenin.

DOI or PMID #

https://doi.org/10.1038/s41418-018-0138-z

Additional Notes

Fig. 3b

Image Gallery

Summary

The Good

The antibody works well as expected.

The Bad

N/A

The Bottom Line

The antibody works.

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