Anti-Ubiquitin Antibody Works Very Well

September 23, 2022

pathology
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Anti-ubiquitin antibody

Catalog Number:

sc-8017

Clone Number:

N/A

HEK293T cells stably expressing control (Ctrl) or USP20 shRNAs were transfected with the indicated constructs. After 48 h, cells were treated with MG132 for 6 h before collecting. IP with Flag-tagged beads was performed and then blotted with the indicated antibodies.The results showed that USP20 deubiquitinated β-catenin.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293 whole cell lysates

Primary Incubation

1/500 dilution 4°C overnight incubation

Blocking Agent

5% milk

Secondary Incubation

1/2000 dilution room temperature 1 h incubation

Tertiary Incubation

Room temperature 10 min incubation

Detection

ECL

Results Summary

HEK293T cells stably expressing control (Ctrl) or USP20 shRNAs were transfected with the indicated constructs. After 48 h, cells were treated with MG132 for 6 h before collecting. IP with Flag-tagged beads was performed and then blotted with the indicated antibodies.The results showed that USP20 deubiquitinated β-catenin.

DOI or PMID #

https://doi.org/10.1038/s41418-018-0138-z

Additional Notes

Fig. 3c

Image Gallery

Summary

The Good

The antibody works well as expected.

The Bad

N/A

The Bottom Line

The antibody works very well.

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