BD Biosciences
BUV395 Rat Anti-Mouse IL-17A
565246
TC11-18H10 (RUO)
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CD4 cells differentiate into a range of specialized effector subsets in different healthy and pathological contexts. In mouse models of aging and autoimmune diseases, we study the plasticity and effector functions of the CD4 T cells. We analyzed the production of IL-17 by CD4 T cells with an anti-IL-17 BUV395 antibody in a sick NZBxW mouse.
Flow Cytometry
Splenocytes of a sick NZBxW mouse, incubated in complete culture medium at 37 °C with (Restimulated) or without (unstimulated) PMA/ionomycin for 4h and brefeldin A for the 3 last hours.
Washed twice in PBS and incubated with L/D fixable aqua dye 20 min at 4°C, then washed twice in PBS.
2.4G2
Surface staining with anti-CD3 BUV805, anti-CD4 BUV496, CD44 BV785 etc., in Cell Staining Buffer (Biolegend) incubated 30 min at 4°C.
Cells were fixed and permeabilized with commercial kit (BD Biosciences) for 30 min at 4°C with fixative, 15 min at 4°C with permeabilization buffer, and stained for cytokines and anti-IL-17 BUV395 1/100 in permeabilization buffer.
UV laser on Symphony cytometer
IL-17-producing CD4 T cells could be identified and quantified in stimulated cells, and not in unstimulated cells.
N/A
Bright and specific staining.
It is expensive.
Excellent option to expand a panel with the BUV laser!